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Explore the universe and discover our home planet with the official NASA Tumblr account

Ever want to ask a real life astronaut a question? Here’s your chance!

Astronauts Drew Feustel Ricky Arnold will be taking your questions in a Video Answer Time session. We’ll collect your questions and send them to space to be answered by the astronauts on Friday, May 18. We’ll record their answers and post them on Wednesday, May 23 here on NASA’s Tumblr. Make sure to ask your question now by visiting http://nasa.tumblr.com/ask !

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l was selected by NASA in 2000. He has been assigned to Expedition 55/56, which launched in March 2018. The Lake Orion, Michigan native has a Ph.D. in the Geological Sciences, specializing in Seismology, and is a veteran of two spaceflights. Follow Feustel on Limited Edition Sale Online Iris amp; Ink Woman Jamie Stretchknit Tank Sky Blue Size XL IRIS amp; INK Authentic Sale 2018 Unisex Discount Inexpensive Original Cheap Price udiipft6Yw
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Richard R. Arnold II was selected as an astronaut by NASA in May 2004. The Maryland native worked in the marine sciences and as a teacher in his home state, as well as in countries such as Morocco, Saudi Arabia, and Indonesia. Follow Arnold on Twitter and Instagram .

And don’t forget to submit your questions by 5 p.m. EDT on Tuesday, May 15 at http://nasa.tumblr.com/ask!

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will be taking your questions in an Answer Time session on Friday, May 5 from 10am - 11am ET here on NASA’s Tumblr. See the questions she’s answered by visiting nasa.tumblr.com/tagged/answertime !

NASA astronautJeanette J. Epps (Ph.D.) was selected as an astronaut in 2009. She has been assigned to her first spaceflight, which is scheduled to launch in May 2018.Her training included scientific and technical briefings, intensive instruction in International Space Station systems, spacewalk training, robotics, T‐38 flight training and wilderness survival training.

Before becoming an astronaut, Epps worked as a Technical Intelligence Officer at the Central Intelligence Agency (CIA).

Born in Syracuse, New York. Enjoys traveling, reading, running, mentoring, scuba diving and family.

July 16, 2018, National University of Singapore

NUS scientists, together with researchers from Nanyang Technological University (NTU) and Imperial College London (ICL), have developed non-invasive biophysical techniques to quantify oxygen concentration and micromechanical properties in bacterial biofilms and understand their real-time responses to environmental changes.

Communities of bacteria, fungi, protozoa or algae that adhere to each other or surfaces are known as biofilms. The biofilms are characterised by a variety of properties not typically found in isolated free-living organisms. One of the emergent properties of biofilms relevant in the context of human health is their increased tolerance to disinfectants and antibiotics. While some biofilms are beneficial (e.g. those involved in wastewater treatment and bioremediation), many others are harmful (e.g. those involved in infections and corrosion). As with any living organism, biofilms continually adapt and respond to a variety of environmental stresses, such as changes in nutrient or oxygen availability.

Oxygen plays an essential role in the generation of energy for cell maintenance and growth. Quantifying the amount of oxygen is necessary to study its effects during the various stages of biofilm growth. Current tools for measuring oxygen levels in biofilms either consume oxygen themselves (leading to less accurate results) or can only obtain accurate measurements from the surface but not within the biofilms.

To overcome these limitations, Prof Thorsten WOHLAND from the Departments of Biological Sciences and Chemistry, NUS together with Prof Yehuda COHEN and Prof Scott RICE from NTU have adapted a non-invasive technique called Transient State (TRAST) imaging and applied it to quantify oxygen levels in bacterial biofilms . This led to the identification of oxygen deficient zones within the microscopic colonies of P. aeruginosa. TRAST is a luminescence based imaging technique. It is based on the fact that certain fluorophores (a type of fluorescent chemical compound) occupy two different states, one which emits fluorescence and the other a non-fluorescent dark state. The fraction of fluorophores in the dark state depends on how often the fluorophores are excited and whether they are given enough time to come back from the dark states to the fluorescent states. By changing the illumination scheme in defined ways, the amount of fluorophores across the biofilm (in the dark state) can be measured easily. The measurements depend only on the fraction of fluorophores in the dark state, meaning that the accuracy is not affected even if certain regions in the biofilm have higher fluorophore concentration. Since oxygen suppresses the occupation of dark states, and thus lowers the fluorophores that reside in the dark state , TRAST can be used to quantify oxygen concentrations.

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